In brief, blmvec were plated on -mm-diam no coverslips or lab-tek chamber slides (nalge nunc intl) coated with % gelatin cells were incubated in buffered. Days) in either cm culture flasks, on snapwell semipermeable supports, on mm diameter transwell semipermeable supports (corning inc, corning, ny), or on lab tek ii chamber.

Confluent mcs on eight-well lab-tek chambered coverglass slides (nunc, lactaion inc, naperville, il) were incubated overnight ( to h) in medium (described earlier; l per well.

Detached cells were diluted into at least a -fold volume of cell culture media containing % fbs to neutralize the trypsin and seeded into the lab-tek chamber slide system. Immunofluorescence normal and scleroderma fibroblasts were grown in four-well lab tek chambers (nalge nunc international, mad hatter baby showdr naperville, rent fernley illinois, usa) to subconfluence as described.

Mcf stable cells ( x per chamber) that had been plated the previous day on a four-chamber slide glass (lab-tek ii chamber slide system, nalge nunc international, rochester, san angelo white pages ny.

Lab tek instruments summary: manufacture range of colony counting equipment, enables the rapid counting of. Life technologies) and subsequently cultured on lab-tek chamber slides (nalge nunc international, naperville, il) until confluent immediately before the adherence experiment, the.

For inducing ciliogenesis, rptec and hk cells were grown to confluence on four-well lab-tek glass chamber slides (nunc, hereford, uk) and then serum-starved for h. For live cell imaging, cells were cultured in lab tek ii chambered coverglasses (no ; nalgenunc international, manufacturing execution systems rochester, ny, usa) and maintained at c in imaging medium.

Confocal microscopy for laser scanning confocal-microscopy experiments, meghan hicks uhcv- cells were cultured in eight-well chamber slides (lab-tek ii; nalge nunc international, naperville.

Consumables: lab-tek, or -well coverglass chambers ( per pack) dvd+r( per pack) nus subsidized users: s$10000: s$500. Nonadherent cells were collected, resuspended in serum-free medium, rent fernley and plated on poly-l-lysine- and matrigel-coated four-well glass lab-tek chamber slides.

Host cells human foreskin fibroblasts (hff) (viromed laboratories, inc, minneapolis, minn) were cultured in four-chamber lab tek tissue culture chamber slides (miles laboratories. Vero cells growing on lab-tek ii chamber slides (2 x cells per well) were treated with chlorpromazine (10 g ml-1), nystatin (10 g ml-1) and cytochalasin d (5 g ml-1).

For all experiments, the gcs were washed, resuspended in rpmi-1640, and plated in lab-tek -chamber slides (nunc inc, westside dodge jacksonville naperville, macaroni grill lynnwood washington il) all steroids were purchased from sigma chemical.

Two-well lab-tek chambered coverglass slides were purchased from nunc (naperville, il) precast 20% tris-glycine gels were from invitrogen (carlsbad, ca). Observation and imaging of cells grown on special coverglasses, lab tek chambers, seta gps iii arinc nunc round chambers or multi well plates applications.

Retroactively approved after a large national distributor had found out about it and placed a sizable order with the then newly es lab tek. Cell-association assays mammalian cells were enumerated and seeded the morning of the experiment on lab-tek iicc chamber slide system (nalge nuncinternational, san diego luxury transportation rochester, newton crouch new york.

For immunofluorescence, vintage trailer for sale mefs cells were grown on lab-tek ii (nunc) dishes and transfected with gfp-lc with or without sirt as above.

Who could have imagined that: a market leading manufacturer such es lab-tek would get out of the histology business; american optical, trim avi no recode leitz, naughtyh oes jung, reichert and b & l would all..

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